Composite

Part:BBa_K3002119:Design

Designed by: Marlene Schlosser   Group: iGEM19_TU_Kaiserslautern   (2019-10-21)


Level 1 – Hygromycin resistance + Tub2 Promoter + Tub2 Terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1408
    Illegal XhoI site found at 250
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 773
    Illegal NgoMIV site found at 955
    Illegal NgoMIV site found at 1326
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is codon-optimized for Chlamydomonas reinhardtii. Additionally, it is compatible with Phytobricks /MoClo parts.


Source

This part is a combination of the parts BBa_K3002011 (Tub2-promoter), BBa_K3002012 (Tub2-Terminator), BBa_K3002013 (CDS of hygromycine resistance), , BBa_K3002303 (connector B2-B3) and BBa_K3002305 (connector B5-B6).

References